目的 建立了粉花绣线菊 Spiraea japonica L.f.愈伤培养系统和快速繁殖方法。方法 通过用 MS、6,7-V、B5等 3种不同的培养基对粉花绣线菊植株的茎尖、嫩叶、叶柄等不同部位进行愈伤组织诱导和成苗诱导。结果 通过本研究,建立了愈伤培养系统,实现了快速繁殖。结论 MS培养基诱导愈伤效果最好,在 MS+2,4- D2 .0mg/L +KT 0 .3m g/L培养基中,幼叶、茎尖、叶柄等外植体均能诱导出愈伤组织,其中以茎尖外植体诱导的效果最好 ;茎尖外植体在 MS+2 mg/L BA+0 .1mg/L NAA能长出丛生苗,将丛生苗转入培养基 1/2 MS+0 .2 5 mg/LBA+0 .5 m g/L NAA可生根成小苗.

Object To establish a calli culture system for the rapid propagation of Speiraea japonica L. f. Methods Callus and shoot induction were carried out on MS, 6, 7 V or B 5 media with different parts of the plant such as stem tip, tender leaves and petiole as explants. Results A calli culture system was established for the rapid propagation of S. japonica. Conclusion MS cultural medium was found to be most suitable for calli induction. MS with 2.0 mg/L 2,4 D+0.3 mg/L KT can induce calli when the explants were used for the induction, with stem tips being the most satisfactory explant. Clusters of seedlings can be induced on MS+2 mg/L BA+0.1 mg/L NAA and when these seedlings were transferred to 1/2 MS+0.25 mg/L BA+0.5 mg/L NAA medium, root were developed to give young seedlings.

国家自然科学基金(30070087)资助;中国科学院资源与生态环境研究重点项目(KZ952-31-108)经费资助