目的 测定麻黄汤分煎、合煎汤剂中甘草酸含量。方法 采用 HPLC法测定麻黄汤分煎与合煎液中甘草酸的含量。选 Hypersil C₁₈柱,流动相 :乙腈 - 0 .1%乙酸水溶液 (33:67),检测波长 254nm。结果 分煎液甘草酸平均回收率为 102 .43%,RSD=2 .65% ;合煎液甘草酸平均回收率为 99.41%,RSD=3.11%。结论 方法简便、准确,复方中其它成分对测定无干扰.

Object To determine the content of glycyrrihizic acid obtained when each individual ingredient in MAHUANG DECOCTION was decocted separately and then mixing the extracts with boiling water in comparison with that obtained by decocting the total composition together as a whole in the traditional way. Methods The contents of glycyrrhizic acid was determined by HPLC. Hypersil C₁₈ column was used, with acetonitrile∶0.1% acetic acid (33∶67) as the mobile phase and detected at the wavelength of 254 nm. Results The average recovery of glycyrrhizic acid when separately decocted was 102.43%, RSD=2.65%, while that of decoction in whole was 99.41%, RSD=3.11%. Conclusion The method was simple and accurate and was not interfered by other constituents in the prescription.

国家自然科学基金资助项目(39960083)