目的 建立墨旱莲 Eclipta prostrata的毛状根离体培养系统。方法 分别用发根农杆菌 A4,R16 0 1,ATCC15 8343种菌株感染墨旱莲的子叶外植体 ,获得毛状根 ,并筛选了优质株系 ;测定了毛状根的生长曲线 ;利用高压纸电泳法对毛状根进行 T- DNA转化的检测。结果 首次利用发根农杆菌 A 4,R16 0 1,ATCC15 8343种菌株成功地从墨旱莲中诱导出毛状根 ;经高压纸电泳检测 ,墨旱莲毛状根中含有甘露碱 ,表明 Ri质粒的 T- DNA已整合进毛状根中。结论 墨旱莲毛状根离体培养的建立为进一步进行药用活性成分的工业化生产奠定了基础

Object To establish the culture system of hairy root of Eclipta prostrata (L.) L.. Methods Hairy root of E. prostrata was obtained from infected cotyledon explants after infection with Agrobacterium rhizogenes strains A4, R1601 and ATCC15834, elite strains were screened and growth curves determined. The transformation of Ri T DNA was examined through high voltage paper electrophoresis. Results The hairy root was originally obtained from E.prostrata. The result of high voltage paper elctrophoresis confirmed the transformation of T DNA from Ri plasmid to the hairy root. Conclusion The acquisition of hairy root of E. prostrata provided further a foundation for the industrial production of active drug component.