目的 建立远志药材的质量控制方法。方法 采用HPLC法对远志中去羟基远志皂苷元进行含量测定，ODS Hypersil色谱柱(5μm，125mm×4mm)；流动相：甲醇－0．05%磷酸溶液(74∶26)；检测波长210nm；结果 去羟基远志皂苷元在0．585～11．7μg范围内与峰面积呈良好的线性关系，r=0．99999；平均回收率为99．21%(n=5)，精密度的RSD为0．25%(n=5)。结论 该方法简便、准确、重现性好，可作为远志药材及其复方制剂质量控制标准。

Object To establish an HPLC method for the determination of dehydroxypresenegenin for the quality control of Polygalae tenuifolia Willd. Methods Dehydroxy presenegenin was separated by ODS column (125 mm× 4 mm, 5μm) with a mobile phase of methanol-0.05% phosphoric acid (74∶26) and detected at a wavelength of 210 nm. Results The calibration curve was linear in the range of 0.585～11.7μg, with a correlation coefficient of 0.999 99. The average recovery was 99.21% (n=5) , and RSD= 0.25% (n=5). Conclusion The method is simple and accurate, with good repeatability and can be used for the quality control of Polygalae tenuifolia Willd and compound preparations of Radix Polygalae.

“九·五”国家重点科技攻关资助项目 (No．95-903-04)