目的 研究中药大黄有效成分大黄素对不同状态下的巨噬细胞分泌TNF_α、IL-1、IL-6和[Ca²⁺]_i的影响。方法 利用脂多糖 (lipopolysacchride, LPS)刺激的大鼠腹腔巨噬细胞作为过度炎症反应的体外模型, 用生物学方法和荧光分光光度法测定巨噬细胞合成分泌的TNF_α、IL-1、IL-6和[Ca²⁺]_i。 结果 大黄素对 TNF_α 的分泌和[Ca²⁺]_i有双向调节作用。 结论 大黄素可能具有免疫双向调节功能。

Object To study the effect of emodin, the active principle in rhubarb on the secretion of TNF_α, IL-1, IL-6, and [Ca²⁺]_i by rat peritoneal macrophage under different conditions. Methods Hyper-nomic inflammation models of isolated rat peritoneal macrophage were prepared by lipopolysaccharide exci-tation and the levels of [Ca²⁺]_i and the proinflammatory cytokines TNF_α, IL-1 and IL-6 secreated deter-mined by fluorescence spectropho to metry and bioassay. Results Emodin showed adual regulatory effect on the secretion of proinflam matory cytokines and[Ca²⁺]_i. Conclusion It seemed that emodin has a biphasic immuno-regulatory effect.