目的 分离出大黄中4种有效成分:大黄酚、大黄素、土大黄苷和大黄酸。方法 采用胶束电动毛细管色谱法(MECC), 缓冲溶液为50mmol/L H3BO₃-NaOH含20mmol/L SDC, pH值为10, 分析电压17kV。 结果 4种成分在5min内全部分离。 结论 本法简便、快速、结果令人满意。

Object To isolate and identify the four active constituents, chrysophanol, emodin, rhaponticin and rhein in rhubarb. Methods Micellar electrokinetic capillary chromatography(MECC ) with a buffer solution of 5 mmol/L H₃BO₃-NaOH containing 20 mmol/L of sodium deoxycholate(SDC)(pH=10) was used for their separation at a voltage of 17kv. Results The four active constituents were completely separated within 5 min. Conclusion This method was found to be simple and rapid and gave satisfactory results.

国家自然科学基金(29675022,29875027), 国家“九·五”科技攻关项目(96-A 23-01-06)和中科院长春应用化学研究所电分析化学开放研究实验室基金(OC 2000001)资助项目