[关键词]
[摘要]
目的 比较藏药“雪莲”原植物水母雪莲及其混淆种类的 ITS序列 ,为分子序列鉴定提供参照系统。方法 利用 PCR扩增和 ABI377自动测序。结果 水母雪莲不同居群间的序列相同 ,与其混淆种类之间存在一定的分子序列差异。结论 ITS序列可正确鉴定水母雪莲与其混淆种类。
[Key word]
[Abstract]
Object In order to identify the medicine at the molecular level, the internal transcribed spacers (ITS) of Saussurea medusa Maxim and its easily confusable species were sequenced Methods The double stranded DNA was amplified using PCR systems 9 600 kits and sequenced on an ABI 377 automated sequencer from both directions Results The ITS sequences of S medusa of different populations showed no variation, but there existed distinct variation between S medusa and its confusable species Conclusion ITS sequences can be used for the molecular authentication between S medusa and its confusable species
[中图分类号]
[基金项目]
中国科学院生物科学与生物技术特别支持费课题资助