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[摘要]
目的 观察绞股蓝总皂苷 (GP)对全脑缺血再灌注大鼠海马及齿状回 DNA和 RNA的保护作用。方法 采用 4-血管阻断 (4- VO)方法建立大鼠急性全脑缺血模型 ,用吖啶橙染色法进行 GP对全脑缺血再灌注大鼠海马及齿状回的 DNA和 RNA保护作用的研究。结果 与正常对照组比较 ,全脑缺血再灌注大鼠海马及齿状回 DNA和RNA吖啶橙染色后的荧光强度 (反映 DNA和 RNA含量 )明显减弱 ,GP10 0 mg/kg ig给药组海马及齿状回 DNA和 RNA吖啶橙染色后的荧光强度强于全脑缺血再灌注模型组 ,与正常对照组相似。结论 GP可明显减轻缺血再灌注对大鼠海马及齿状回 DNA和 RNA损伤
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[Abstract]
Object To observe the effect of gypenosides (GP) on DNA and RNA in hippocampus and dentate gyrus of cerebral ischemia reperfusion injured rat Methods Modified 4 vessel occlusion (4 VO) method was used to establish the models of acute global ischemia Acridine orange (AO) method was carried out to study the protective effect of GP on cerebral ischemia reperfusion injury in hippocampus and dentate gyrus of rat Results The fluorescent intensity of the DNA and RNA in hippocampus and dentate gyrus of cerebral ischemia reperfusion injury of rat is abated as compared with the normal control while the group with ig GP (100 mg/kg) is enhanced when compared with the ischemia reperfusion injury model, and showed the same degree of intensity with the normal control group Conclusion It is shown that the injury on DNA and RNA in hippocampus and dentate gyrus of ischemia reperfusion of rat could be alleviated by GP
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