目的 研究 HPL C测定条件 ,并测定不同干燥方法贯叶连翘中黄酮类化合物芦丁、金丝桃苷的含量。方法 色谱柱 :Discovery C1 8(5μm ,2 5 cm× 4.6 mm ) ;流动相 :水 -乙腈 -磷酸 (82 5∶ 175∶ 1) ;流速 :1.0 m L / m in;检测波长 :36 5 nm。结果 6 0℃烘干 4h贯叶连翘中芦丁和金丝桃苷的含量最高 ,总含量可达到 1.6 77%。芦丁的线性范围为 0 .10 7～ 2 .6 75 μg,平均回收率为 99.32 % ,RSD=1.0 0 7% ,金丝桃苷的线性范围为 0 .10 7～ 2 .6 75 μg,平均回收率为 99.5 4% ,RSD=3.5 91%。结论 该方法分析简便、灵敏、迅速、准确

Object To develop a method for the determination of flavonoids in Hypericum perforatum L processed by different drying Methods to provide a basis for the processing of the natural herbs Methods The chromatographic conditions were Discovery C 18 column (5 μm, 4.6 mm × 25 cm). detection wavelength 365 nm; mobile phrase : water : acetonitrile : phosphoric acid (825∶175∶1); flow rate 1.0 mL/min Results The contents of rutin and hyperin were at their maximum when dried at 60 ℃ for 4 h Rutin had a good linearity in the range of 0.107～2 675 μg, average recovery rate 99 32%, RSD 1 007%, and hyperin in the range of 0 107～2 675 μg, average recovery rate 99 54%, RSD 3 591% Conclusion Temperature is the main factor influencing the content of flavonoids The HPLC determination was shown to be rapid, reliable and simple, and may be used for the quality control of H perforatum