目的 利用毛细管电泳法鉴别天麻及其 4种常见伪品以及测定天麻中有效成分天麻素。方法 电泳分离条件为 2 4m mol/L 硼酸盐 (p H=9.4)、运行电压为 16 k V。结果 在该分离条件下,通过比较电泳谱图和天麻素加标实验,4种伪品与天麻可以很容易鉴别 ;天麻中的天麻素可以达基线分离,定量测定在 15 m in之内完成,线性相关方程为 Y=- 0 .0 48+7.79X(r=0 .999)。结论 方法简便、快速、准确,可以作为中药天麻的质量控制方法

Object To develop a method for the distinction of genuine Gastrodia elata B1. from its four falsified plants of common occurrence and determination of its active principle, gastrodin by capillary electrophoresis. Methods The electrophoretic conditions were a buffer solution containing 24 mmol/L borate (pH=9.4), at a voltage of 16 kV. Results The electrophorograms obtained under the above conditions showed easily distinguishable differences. Baseline separation of gastrodin was achieved and its quantitative analysis can be accomplished within 15 min. The linear correlation equation was Y=-0.048+7.79X (r=0.999). Conclusion This method could serve as an easy and precise method for the quality control of G. elata.Keywords：capillary electrophoresis; Gastrodia elata Bl.； gastrodin

河北省自然科学基金资助项目(200077)