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[摘要]
目的 建立黄芪粉针剂的质量控制方法。方法 采用薄层色谱 -分光光度法对黄芪粉针剂中的主要成分黄芪甲苷进行含量测定,展开剂为氯仿 -甲醇 -水 (6 5∶ 30∶ 10 ),测定波长为 418nm。结果 回归方程 A=1.5 38C+0 .189,r=0 .9998,在 80~ 2 40μg点样量范围内,吸光度与点样量呈良好的线性关系,平均回收率为 98.6 8%,RSD=2 .5 2 % (n=5 )。结论 本方法使用简单仪器测定,结果可靠,专属性强,可作为黄芪粉针剂的质量控制标准
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[Abstract]
Object To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results The regression equation within the spotted range of 80~240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.
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