目的 研究油茶皂苷 (SQS)预适应保护作用及 NO的关系。方法 以 ISO诱发大鼠心肌缺血损伤为模型 ,使用特异性 NO合成抑制剂 Methylene blue(MET)测定大鼠 ECG及 CPK活性 ,FFA和腺苷含量。结果 预适应iv SQS能有效地保护 ISO所致大鼠心肌损伤 ;先 iv MET后再给予 SQS,则 SQS对心肌损伤的保护作用明显减弱。结论 SQS对 ISO所致心肌缺血大鼠可产生药理性预适应保护作用 ,该作用由 NO所介导。

Object To study the protective effect and pharmacological ischemic preconditioning of sasanquasaponin (SQS) on intact rat heart and its relationship with NO. Methods A model of rat myocardial ischemia induced by sc injection of isoproterenol (ISO) was prepared, and after the administration of selective NO synthesis inhibitor methylene blue (MET), the ECG, serum creatine kinase (CK) activity, free fatty acid (FFA) and adenosine contents were determined. Results Preconditioning iv injection of SQS can effectively protect the myocardium from ischemia induced by ISO. The cardio protective effect was significantly attenuated when the NO synthesis inhibitor MET were injected prior to SQS. Conclusion SQS can effectively protect myocardium ischemia induced by ISO and the protection is most likely to be mediated by NO.

江西省自然科学基金资助!(C950102)