目的:对不同品种的诃子进行DNA指纹图的鉴别研究,并对RAPD在中药材鉴别中存在的一些问题进行研究。方法:应用RAPD技术对诃子及其常见混淆品进行DNA指纹图多态性的比较。结果:DNA指纹图显示,各样品间具有不同的遗传关系,根据这些差别可进行鉴别,同时对存在的若干问题提出了解决的方法。结论:RAPD可以有效地鉴别诃子及其混淆品

Different varieties of Terminalia chebula Retz were identified by DNA finger prints, and at the same time, some problems encountered during the application of RAPD for the identification of TCM were studied The polymorphic features of DNA finger prints of different species were compared Results of the study showed that they were differently related in heredity which can provide a basis for the differentiation of one species from the other Means for the solution of problems arised during RAPD application were suggested It was concluded that RAPD is an effective method to identify T chebula from its confusable varieties

国家中医药管理局科研基金!(95 0 379) ;广东省五个一工程科研基金