对香菇多糖提取、纯化条件进行优化研究,结果表明:香菇多糖提取最佳工艺条件为:pH7.0,96℃浸提4h,料水比为1∶20,醇析浓度为70%,蛋白质去除时样品-氯仿+正丁醇(V∶V)为1∶1,氯仿-正丁醇(V/V)为1∶0.25,萃取时间为30min效果最好。不同类型的香菇原料的提取结果表明:椴栽和袋栽香菇、花菇和板菇,其香菇多糖含量相差不大。从经济角度和工艺的简易性考虑,分离制备香菇多糖应以袋栽板菇为最佳

Conditions for the extraction and purification of lentinan from mushroom were optimized. Result of the optimization showed that extraction with 20 times of water at 96 ℃ for 4h with pH adjusted to 7.0 gave the best result. Lentinan could be obtained by adding with 70% ethanol to an ultimate concentration of 70% and made protein free with a mixed solvent of 1∶0.25 chloroform∶n butanol. All natural or artificially cultivated lentinus edodes gave nearly identical yields, but from the point of economy and simplicity, artificially cultivated non brindled lentinus edodes seemed to be the best choice.

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