[关键词]
[摘要]
目的:板蓝根二酮B抗人肝癌BEL-7402细胞、卵巢癌A2780细胞体外活性研究。方法:MTT法测定板蓝根二酮B对肝癌BEL-7402细胞和卵巢癌A2780细胞的抑制作用,集落形成实验观察药物的诱导分化作用,PCR-ELISA试剂盒测定细胞的端粒酶活性。结果:板蓝根二酮B具有抑制肝癌BEL-7402细胞、卵巢癌A2780细胞增殖的能力,其IC50分别为8.2μg/mL和7.8μg/mL;且具有诱导分化作用,可降低端粒酶活性的表达。结论:研究结果提示板蓝根二酮B具有体外抗肿瘤活性。
[Key word]
[Abstract]
To study the in vitro anticancer activity of tryptanthrin B, one of the active constituent of the root of Isatis tinctoria L on BEL-7402 human hepatocellular carcinoma cell and A2780 human ovarian cancer cell Cytotoxicity assay was tested by MTT method Signs of induction differentiation was observed by colony forming units assay Telomerase activity in cell was determined by PCR-ELISA method IC 50 of tryptanthrin B on BEL-7402 and A2780 cell lines were 8 2 μg/mL and 7 8 μg/mL respectively Telomerase activity was dramatically declined and defferentiation of both cancer cell lines was observed Tryptanthrin B possesed antitumor activity in vitro
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[基金项目]
广西教育科研资助 项目 ;桂 1999[383] -5