探讨了银杏内酯B对体外血小板活化因子激活的大鼠中性白细胞某些功能,包括溶酶体酶的释放,超氧阴离子的产生以及细胞内游离钙浓度改变的影响。结果显示:银杏内酯B在0.5～100μmol/L终浓度下,可使β-葡萄糖苷酸酶释放量降低44.2%～73.2%;在0.1～40μmol/L终浓度下,可使超氧阴离子生量降低27.4%～50.0%;在0.1～100μmol/L终浓度下,使PAF和fMLP刺激引起的细胞内游离钙浓度分别降低13.9%～51.4%和2.2%～36.6%。结果提示:抑制溶酶体酶的释放、超氧阴离子生成以及降低细胞内游离钙浓度的升高可能与其抗炎作用有关。

100050) Zhou Long'en, Wang Wenjie, Bai Jingye and Chen Guifang To investigate the effects of ginkgolide B on release of β-glucuronidase and the production of superoxide anion, as well as intracellular calcium change of rat polymorphonuclear leukocytes activated by platelet activating factor (PAF) β-glucuronidase release was quantitated by enzyme reaction in which phenophthaleinglucuronic acid was used as the substrate, superoxide anion production was determined by cytochrome C reduction, the change of intracellular calcium was measured by fluorescent indicator Fura-2/Am In comparison with control, ginkgolide B at final concentration of 0 5～100 μmol/L inhibited β-glucuronidase release by 44 2%～73 2%; at final concentration of 0 1～40 μmol/L decreased superoxide anion production by 27 4%～50 0% and at final concentration of 0 1～100 μmol/L decreased the rise of intracellular calcium level induced by PAF and fMLP by 13 9%～51 4% and 2 2%～36 6% respectively It is concluded that ginkgolide B can significantly affect the functions of rat polymorphonuclear leukocytes Therefore, its inhibitory effects on lysosomol enzyme release, superoxide anion production and intracellular calcium increase induced by PAF may be related with its antiinflammation action