目的:探求一种合理有效的中药作用于中枢神经系统药效研究的方法。方法:利用体外细胞培养的方法,以3种不同浓度观察血清、中药血清、脑脊液、中药脑脊液对于星形胶质细胞和神经元的影响。结果:苔盼蓝染色和LDH检测证明正常血清和中药血清在3种浓度均出现对细胞的毒害作用,尤以100,200μL为重,且在同一浓度时,两组之间没有明显的差异。而正常脑脊液组和中药脑脊液组与空白对照组相比,未见明显差异,MTT检测证明中药脑脊液组吸光度(A)明显高于其余各组。结论:利用脑脊液进行中药对中枢神经系统的药效观察明显优于血清,提出“脑脊液药理学”的概念;中药可诱导星形胶质细胞产生神经营养作用。

To explore an effective method by which the pharmacological effects of Chinese materia medica (CMM) of the central nervous system (CNS) could be correctly investigated Cerebrospinal fluid (CSF) and serum were taken 1~1 5 h after administration of CMM and added into the astrocyte cell cultrue medium After 48 h, the incubated medium was transferred in proportion to the neuron culture medium Typan blue stainning, LDH and MTT test were performed to observe the drug effect on the rate of neuron survival The serum,but not the CSF, showed certain degree of cytotoxicity on the astrocyte MTT test on the neuron survival showed that the A 492 nm value of the CMM group was higher than that of the other groups The astrocyte medium stimulated by the CSF containing CMM could promote the neuron axon growth Since CSF is better than serum to evaluate the CMM effects on CNS in vitro, it was deemed that the “Cerebrospinal Fluid pharmacology of Chinese Materia Medica” is worth for further study Furthermore, the CMM could induce the neurotrophic effects of astrocytes

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