目的:采用双波长薄层扫法测定洁阴洗液中苦参碱的含量。方法:用反射式锯齿扫描法测定,测定波长λs=520nm,参比波长λR=640nm;展开剂为:氯仿-甲醇-氨水(30∶1∶0.5),显色剂为稀碘化铋钾试液。结果:点样量在0.613～3.064μg范围内呈良好的线性关系,平均回收率为99.54%,RSD为1.35%(n=6)。结论:该法分离效果好,简便、快速、结果准确,适用于该制剂的质量控制。

To determine the content of matrine in Jieyin Xiye, a lotion for cleaning the pudenda, by TLCS Dual wavelength reflection zigzag scanning was performed at λ S=520 nm, and λ R=640 nm A mixed solvent system of chloroform, methanol, and aqueous ammonia (30∶1∶0 5) was used as the developer; the visualizing agent was dilute potassium iodobismuthate TS The average recovery was 99 54% with RSD=1 35% This method was simple and accurate It is suitable for the quality control of the preparation