研究了用反向毛细管电泳分离测定当归中阿魏酸含量的条件。当归饮片用热水提取,电泳缓冲液为Tris-H3BO3(20∶80mmol/L),并加入0.8mmol/L的CTAB作为电渗流改向剂,分离电压为-10.0kV,用安培检测器检测。分析结果每克当归饮片含阿魏酸0.415mg,RSD2.3%,回收率为97.1%

Experimental conditions for the separation and determination of ferulic acid in the root of Angelica sinensis (Oliv ) Diels by capillary electrophoresis were studied Sliced A sinensis was extracted with hot water, and the ferulic acid in the extract was separated by capillary electrophoresis and detected by amperometric detector Buffer consisted of 20 mmol/L of tris (hydroxymethyl) aminomethane and 80 mmol/L of boric acid 0 8 mmol/L of cetyltrimethyl ammonium bromide was added as the counter electroosmotic flow agent Separation voltage was -10 0 kV Result of the study showed that 1 g of the dry herb contains 0 415 mg of ferulic acid (RSD=2 3%, γ=97 1%)

国家自然科学基金 资助项目 (批准号 2 96 75 0 33)