采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型,研究枸杞多糖(LBP)对离体肌肉的收缩能力,耗竭肌肉组织内脂质过氧化物以及乳酸产生的影响。结果表明,6.4～25.6mg-mLLBP可使肌肉收缩持续时间下降(P<0.01);肌乳酸含量下降(P<0.05);对肌肉收缩幅度没有影响;对脂质过氧化物含量无明显影响。提示LBP对离体蟾蜍腓肠肌在电刺激条件下引发的收缩疲劳有促进作用。

The effects of Lycium barbarum polysaccharide (LBP), obtained from Lycium barbarum L., on isolated toad gastrocnemius muscular fatigue induced by electric stimulation were investigated. The duration and amplitude of contraction, as well as the lipid peroxidation products and lactate concentrations of the stimulated muscle were determined. Results showed that, LBP at concentrations of 6.4～25.6 mg-mL, can cause a decrease of the muscular contraction time and lower the content of lactate in the fatigued muscles, but without effect on the amplitude of contraction, and amount of lipid peroxidation products. These results indicated that LBP can potentiate muscular fatigue in vitro induced by electric stimulation.

国家自然科学基金资助课题 (39160029)