[关键词]
[摘要]
在猴头菌(Hericiumerinaceus)菌丝发酵培养液中,加入不同浓度的La(NO3)3,接种培养7d后,测定菌体和上清液的多糖含量,发现0.5mmol-L的La(NO3)3可促进菌丝分泌胞外多糖,发酵液中多糖含量较对照高126.69%;0.75mmol-L的La(NO3)3可刺激菌丝多糖的合成,水提多糖含量比对照高19.4%,碱提多糖含量比对照高4.71%。
[Key word]
[Abstract]
Rare earth elements, such as Lanthanum, at certain concentration may exhibit its specific activity to promote the growth of plants and microbes. But studies on its application to increase the yield of polysaccharide of cultured Hericium erinaceus (Fr. ) Pers. has not been reported in literatures. Different concentrations of La(NO3)3were added to the culture medium of H. erinaceus mycelium. Inoculate a Φ 0.5 cm size, 10 d cell age colony into the fermentation medium containing different concentrations of La(NO3)3and culture for 7 d. Collect the supernatant liquid and determine the polysaccharide content after centrifugings extraction and hydrolysis. It was found that La(NO3)3at a concentration of 0.5 mmol-L can increase the extracellular polysaccharide content to 126.69% of the control, while at a concentration of 0.75 mmol-L, the water soluble polysaccharide content of the mycelium is raised by 19.4%, and increased the sodium hydroxide soluble polysaccharide content by 4.71%. It could be concluded that La(NO3)3at concentrations of 0.5~0.75 mmol-L can promote the yield of polysaccharides in H. erinaceus cultural medium.
[中图分类号]
[基金项目]
国家民委资助项目