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[摘要]
用枳壳 Poncirus trifoliata Raf.实生苗的上胚轴为材料,探索以根癌农杆菌介导的GUS基因在枳壳中的转移,结果表明:抑菌剂选择头孢霉素较好;卡那霉素作为选择试剂,浓度为50mg/L;外植体与农杆菌共培养时间以2~3d为宜。GUS基因瞬时表达检测,80%的外植体呈阳性反应;GUS基因稳定表达检测,抗性植株中,GUS反应呈阳性所占比例为70.0%。
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[Abstract]
The transformation of GUS gene by Agrobacterium-mediation was studied.The explants used for transformation were the epicotyls from Poncirus trifoliata Raf.The results indicated that Cefotaxime used as antibiotics was better than carbenicillin.The concentration of kanamycin used as the reagent of choice was 50mg/L.Shoot regeneration frequency was high when cocultivation time was 2~3 days.Histochemical GUS assay showed that 80.0% of the explants had the transient expression of GUS gene and 70.0% of the resistant plants were GUS-positive.
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