体外培养大鼠皮质神经元,用β-淀粉样蛋白(β-AP)诱导皮质神经元凋亡。黑海参多糖使皮质神经元存活率增加,使神经元的DNA电泳图谱不出现“梯子状”,使神经元的流式细胞仪分析图上不出现凋亡峰,表明黑海参多糖对β-AP诱导的皮质神经元凋亡具有保护作用。

Cortical neurons were cultured in vitro and induced to apoptosis by amyloid β-protein (β-AP).Polysaccharides from several TCM were screened with the apoptotic model.It was found that the polysaccharide obtained from Holothuria atra Jaegar increased the survival rate of cortical neurons in a dose dependent manner,and prevented the neuronal nuclei from shrinkage,condensation and cleavage induced by β-AP.It also prevented neuronal nuclear DNA fragmentation and blocked the neuronal apoptotic peak induced by β-AP.These results indicated that polysaccharide from H.atra can block cortical neuronal apoptosis induced byβ-AP.

由国家杰出青年基金(No.396250022);国家自然科学基金(No.39770851 No.39870265);广东省自然科学基金(No.960125)