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[摘要]
对“大承气汤”颗粒进行了定性定量研究。通过薄层色谱法鉴别大黄、厚朴、枳实 ;反相高效液相色谱法测定方中君药大黄游离型蒽醌和总蒽醌的含量,采用 ODS(3)5μm色谱柱,4.6mm×250 mm,流动相:甲醇-1%高氯酸水溶液(85∶15),流速为1.0mL/min,检测波长:254nm;测定回收率:游离型蒽醌以大黄素、大黄酚为指标,回收率为101.97%,RSD=3.44%,总蒽醌以大黄酸、大黄素、大黄酚、大黄素甲醚为指标,回收率为98.99%,RSD=1.53%。测定结果:3批样品的平均含量,游离型蒽醌为0.0303mg/g,总蒽醌为13.614mg/g。
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[Abstract]
The identity and contents of Radix et Rhizoma Rhei,Cortex Magnoliae Off icinalis and Fructus Aurantii Immaturus in Dachengqitang Granule were determined by TLC.The “ monarch”ingredients including free anthraquinones and total anthraquinones were determined by RP-HPLC.The average recovery rate of free anthraquinones (emodin and chrysophanol) was 101.97%,RSD= 3.44% ;and that of total anthraquinones (rhein,physcion,emodin,etc.) was 98.99%,RSD= 1.53%.
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