应用体外集落与液体培养、细胞形态学观察、DNA片段凝胶电泳及 DNA片段百分率测定等方法观察灵芝复方对人骨髓 CFU- GM生长及 HL- 6 0细胞增殖和凋亡的作用。发现灵芝复方在一定浓度范围内对人骨髓 CFU - GM生长有促进作用,而对白血病细胞系 HL - 6 0细胞集落生长则表现出剂量依赖性抑制 ;细胞形态学结果表明,灵芝复方对 HL- 6 0细胞凋亡具有剂量和时间依赖性诱导作用。 8和 16 mg/ m L 灵芝复方作用48h,可使 HL - 6 0细胞 DNA片段百分率明显升高 ;DNA片段凝胶电泳显示有明显的细胞凋亡“梯形”表现。提示灵芝复方可通过选择性诱导白血病细胞凋亡来发挥抗白血病作用

A series of experiments including semi-solid colony culture and liquid culture in vitro, DNA fragments in gel electrophoresis and the percentage of DNA fragmentation test were undertaken to investigate the effects of Ganoderma lucidum (Leyss ex Fr) Karst Compound (GLC) on the growth of CFU-GM derived from human bone marrow and on the proliferation, apoptosis of HL-60 cells. The results indicated that different concentrations of GLC could promote human BM CFU-GM proliferation, but suppressed the growth of HL-60 cell colonies. The cell morphological observation discovered that GLC induced HL-60 cells apoptosis in a dose-dependent and time-dependent manner. Treating HL-60 cells with 8 mg/mL or 16 mg/mL GLC for 48 h increased the percentage of DNA fragmentation of these cells, and produced typical ladders of DNA fragments in gel electrophoresis. It is concluded that GLC could kill the leukemic cells by inducing them to apoptosis. GLC would be a good medicine for leukemia therapy.

湖南省卫生厅基金