以两头尖皂苷 D(raddeanoside D)为含量测定指标,采用 HPL C法测定了产于黑龙江、吉林、辽宁、山东共 10个药材中皂苷 D的含量,分别为 0 .35 5 0 %、0 .32 13%、0 .2 80 7%、0 .2 196 %,本法快速、简便、重现性好,为两头尖质量控制和评价提供了可靠的依据

Using raddeanoside D, the main active constituent of Anemone raddeana Regel, as the quality control criteria, 10 samples of the herbal drug collected from different localities of Heilongjiang, Jilin, Liaoning and Shandong provinces were analysed by HPLC. Results showed that the average raddeanoside D contents were 0.355 0%, 0.321 3%, 0.280 7% and 0.219 6% respectively. This method was proved to be quick, simple and reproducible, and provided a reliable basis for the quality control and evaluation of A.raddeana.

国家“九·五”攻关项目“中药材质量标准规范化研究”资助项目