为了研究瓜蒌皮治疗冠心病的有效成分,用醚醇法和水煎醇沉法提取瓜蒌皮的有效成分,并用⁴⁵Ca跨膜测量方法研究各提取物对大鼠主动脉PDC钙通道Ca²⁺内流的影响。发现氯仿和醇溶物能阻滞Ca²⁺内流,水溶物具有相反的作用,其中氯仿提取物浓度为30 mg/mL时,使Ca²⁺内流量比对照下降31.57%。实验结果表明瓜蒌皮中可能有7种组分具有治疗冠心病的活性。

Powdered mesocarp of Trichosanthes kirilowii Maxim. was extracted with petroleum ether, ethanol and water, partitioned with chloroform and ethyl acetate or precipitated with the addition of ethanol to obtain a total of 14 fractions. Their effects on Ca²⁺ influx of rat aortic rings were studied with ⁴⁵Ca transmembrane influx technique. Results showed that 7 fractions could block potential dependent Ca²⁺ influx, suggesting their potential use for the treatment of coronary heart diseases. Among them, chloroform extract resulted in a decrease of Ca²⁺ influx of 31.57% in comparison with the control.

国家自然科学基金19775034;核工业科学基金