采用 RP-HPLC方法对黄花乌头 Aconitum coreanum抗心律失常有效成分关附甲素(guanfu base A)的含量进行了测定。并对不同采收期黄花乌头的不同部位中关附甲素的含量进行比较。结果显示关附甲素在8月份采收的根中含量最高。实验采用C₁8柱,以乙腈-磷酸三乙胺缓冲液为流动相,检测波长208nm,以外标法定量,为合理有效地利用药物资源提供了参考。

A method for the determination of guanfu base A, the active anti-arrhythmic principle in Aconitum coreanum Raipaics by RP-HPLC was reported. Results showed that its content was higher in the root collected in August. A good seperation and quantitative determination method was accomplished on an ODS column with UV detector at 208 nm. CH3CN/H2O-H3PO4-NEt3 (2∶8 v/v, pH 3.04) was used as the mobile phase.