利用铺有 Matrigel的 Boyden小室观察抗CD9、抗CD42a、抗CD 63、抗TSP抗体对PG-CL3细胞侵袭作用的影响;采用流式细胞术观察2.5、10、20mg/L的川芎嗪、丹参酮ⅡA和50、500、1000 U/I.的水蛙素和凝血酶对 PGCL3细胞膜表面 CD9、CD42a、CD63、TSP表达的影响,观察川芎嗪、丹参酮ⅡA、水蛭素和凝血酶对人肺巨细胞癌PGCL3细胞表面血小板免疫相关抗原CD9、CD42a、CD63和TSP表达的影响,部分阐述各种药物对肿瘤粘附和侵袭影响的不同作用机制.结果抗CD9抗体明显增加 PGCL3细胞的侵袭,而抗CD42a、抗CD63、抗TSP抗体不同程度减少PGCL3细胞的侵袭.川芎嗪可使CD9+、CD42a+、TSP+细胞的数目减少,PGCL3细胞表面CD9的表达减少;丹参酮ⅡA使CD9的表达有所增加,CD63和 CD42a的表达有所下降,CD42a+、TSP+的细胞数目下降;水蛭素使CD9、CD63和CD42a的表达有所减弱,CD42a+、CD63+细胞的比例下降;凝血酶使CD9和CD42a的表达有所减弱,并使CD42a+的细胞数目减少.提示川芎嗪、丹参酮ⅡA、水蛭素和凝血酶对PGCIJ3细胞表面表达的血小板免疫相关抗原CDg、CD42a、CD63、TSP的不同影响可能与它们对PGCI,3细胞粘附和侵袭的不同作用有关.

Effects of tetramethylpyrazine(I),tanshinoneⅡA(Ⅱ),hirudin(Ⅲ)and thrombin(Ⅳ)on the expression of CD9,CD24a,CD63 and TSP on human pulmonary giant cell carcinoma(PGCL3)were investigated by Boyden Chamber covered with Matrigel with the aid of flow cytometry.Results showed that anti-CD9 antibody significantly augmented,while the other three antibodies(anti-CD42a,anti-CD63 and anti-TSP),impaired the invasive potentials of PGCL3.I decreased the expression of CD9 and the number of CD9+, CD42a+ and TSP+ cells;Ⅱ increased the expression of CD9,but decreased the expression of CD63 and CD42a as well as the number of CD42a+ and TSP+cells;Ⅲ decreased both the expression of CD9,CD63 and CD42a and the nu-mber of CD42a+ and CD63+ cells 4 and Ⅳ decreased the expression of CD9,CD42a and the number of CD42a+ cells.The different effects of these drugs on the expression of antigens immunologically related to platelet on PGCL3 cell surface may be related to their different influential actions on the adhesion and invasion of PGCL3 cells.

国家自然科学基金, 39670912