对中医治疗色素增加性皮肤病方剂69首进行计算机拆方排序,选出高频次出现中药82 味,观察这些中药乙醇提取物对蘑菇酪氨酸酶和无细胞系统多巴色素自动氧化生成黑素量的影 响.结果显示11味中药乙醇提取物在3个不同浓度对酪氨酸酶活性和黑素生成量呈剂量依赖性 抑制,其中白术、白僵蚕、藁本、白芨、白附子、沙苑子、六月雪、柿叶对酪氨酸酶活性抑制率与单体 化合物熊果苷无统计学差异(P>0.05).进-步研究这些中药的皮肤脱色机制与评估其临床应用 价值是必要的.

82 Chinese crude drugs were selected according to their frequencies appearing in 69 TCM prescriptions for the treatment of hyperpigmentory disorders.Their ethanolic extracts were tested seperately for their respective activity to inhibit mushroom tyrosinase and melanin biosynthe-sis in cell free systems.Among them,11were found to be active in the inhibition of tyrosinase and the production of melanin from dopachrome by autoxidation in dose dependent manners.The inhibitory effects of Atractylodes macrocephala,Bombyx mori,Ligusticum sinense,Bletilla striat-a,Typhonium giganteum,Astragalus complanatus,Serissa serissoides and Diospyros kaki were either superior or similar to arbutin,though statis-tically nonsignificant(P>0.05).These results suggested that some of the crude drugs could be considered as candidates to whitenthe sk-in,but their mechanisms of action and clinical evaluation need further extensive studies.

国家自然科学基金, 39570658