建立了测定苦参碱和氧化苦参碱含量的高效毛细管电泳法.20 mmoL/L的磷酸盐缓冲液(pH=8.0)为电泳电解质,电压为30kV(运行电流为100～110uA),检测波长为214nm,内标氨茶碱.苦参碱 0.126～1.26 mg/mL、氧化苦参碱 0.10～1.0 mg/mL其浓度与峰面积之比呈良好的线性关系,r均为0.9998,日内、日间变异系数均小于5.7%,常用22种药物除地塞米松外其余的对其无干扰.用本法测定了2批苦参提取物,其中苦参碱为(45.19±0.058)%,(62.81±0.123)%;氧化苦参碱为(8.71±0.03) %,(6.81±0.086)%.结果表明,该方法简单、灵敏,特异性和重现性良好,具有较强的实用价值.

A method has been developed for the determination of matrine and oxymatrine by high performance capillary electrophoresis (HPCE).Aminophy-lline was used as internal standard.Complete separation was achieved with 20 mmol/L running phosphate buffer (pH=8.0) at a constant voltage of 30 kV (current of 100~ 110 PA) and detected at a wavelength of 214 nm.Good linearity was shown between the concentration and peak area in the concentration range of 0.126-1.26 mg/mL for matrine and 0.10~ 1.0 mg/mL for oxymatrine,r=0.9998.The relative standard deviation (RSD) for within-day and between-day was less than 5.7 %.22 different drugs in common use except dexamethasone showed no interference.Two samples of extract of Sophora flavescens Ait.gave results of (45.19±0.058) % and (62.81±0.123) % for matrine and (8.71±0.03) % and (6.81± 0.086)% for oxymatrine on a trial determination.It seemed that the HPCE method is faster,more convenient and economical as compared with HPLC.