采用超临界流体萃取法(SFE)提取中药何首乌中的蒽醌类成分,并用RP-HPLC法进行分离测定.在SFE中,通过对萃取条件的考察,确定萃取压力为42MPa,温度70℃,改性剂量0.4mL,静态萃取时间5min及动态萃取体积5mL.RP-HPLC中,以C₈为固定相,甲醇-0.2%磷酸溶液(70:30)为流动相,检测波长437nm.本法准确可靠,何首乌中各成分的平均回收率分别为大黄酸99.1%(RSD 2.0%)、大黄素 98.2%(RSD1.1%)、大黄酚 100.7%(RSD 2.6%)和大黄素甲醚99.5%(RSD 0.9%).

Supercritical fluid extraction(SFE) was used to extract anthraquinones in Polygonum multiflorum Thunb.and contents of rhein,emodin and physcion in the extract were determined by RP-HPLC.SFE conditions: pressure 42 MPa,temperature 70℃,0.4 mL methanol as modifier,static extraction 5 min and dynamic extraction volume 5 mL.HPLC conditions: the HPLC column was packed with C_8(5um);the mobile phase was methanol: 0.2% phosphoric acid (70:30);the detection wave-length was 437 nm.The method was accurate and reliable.The mean recovery was 99.1%(RSD 2.0%) for rhein,98.2% (RSD 1.1 % ) for emodin,100.7% (RSD 2.6% ) for chrysophanol and 99.5% (RSD 0.9% ) for physcion.