介绍了南方红豆杉植物愈伤组织诱导和继代培养条件，在MS－培养基上，附加不同种类和浓度配比的激素，在光照条件下对植物愈伤组织的诱导率和生长量等进行了比较，其中当2,4-D1.2mg/l+6-BA2.8mg/L=NAA3mg/L或 4mg/L愈伤组织诱导率和生长量最高，结果表明，不同激素组合对愈伤组织诱导率、生长量和发生的性状有明显的影响。为南方红豆杉植物细胞悬浮培养建立了基础，为解决红豆杉的资源贫乏提供了新途径。

Studies on tissue culture of Taxus chinensis var.mairei(Lemee et Levl.) Cheng L.K.Fu was carried out. Results indicated that the rate of callus inducement and yield of paclitaxel in medium MS plus 1.2 mg/L 2,4-D, 2.8 mg/L 6-BA and 3.0 mg/L or 4.0 mg/L NAA were higher than others. The experiments were conducted under light during a 60-day growth period, at temperature in the range of 23±2 ℃. The result provides the possibility of cell culture of Taxus chinensis var.mairei (Lemee et Levl.) Cheng L. K. Fu and also paves a new alternative way for the production of paclitaxel.

陕西省自然科学基金资助项目