用流式细胞仪技术和MTT法观察了云芝糖肽(PSP)对由植物血凝素(PHA)诱导的人外周血淋巴细胞(PBL)增殖和T细胞亚群变化的调节作用.结果表明,50~100μg/mL PSP对经PHA诱导的人 PBL增殖有显著的促进作用(P<0. 05),该浓度的PSP也能促进细胞从G₁期进入S期,并可使CD₄⁺细胞明显升高,使CD₄⁺/CL₈细胞比例增加,提示PSP具有免疫促进作用

Regulatory effect of polysaccheriopeptide (PSP) on human peripheral blood lymphocyte (PBL) proliferation and T lymphocyte subpopulation was studied.The results showed that: phytohemagglutinin (PHA)-activated human PBL could increase proliferation especially after treated with PSP in concentrations of 50～100μg/mL. In this study, the effect of PSP on cell cycling induced by PHA was investigated, DNA synthesis measured by a flowcytometer was shown to be increased but not in a dose-dependant manner. Cell cycling analysis demonstrated that the increment was mainly at the stage when cells enter S phase.It was observed that in PSP treated cases CD+4 cell group increased, suggested that PSP has certain immunoregulatory effect in improving proliferation of activated human PBL, and increasing the CD+4 cell group.