反应-分离耦合技术是红豆杉细胞培养生产紫杉醇的关键技术。作者采用两液相培养技术，深入研究有机溶剂的种类、浓度、加入时间和相毒性对红豆杉细胞悬浮培养细胞生长和产生的影响。结果表明油酸和邻苯二甲酸二丁酯适合作此体系两液相培养的有机溶剂，合适的浓度为8%，加入时间为第7日～10日，与对照组相比，两液相培养的紫杉醇产量提高4倍。

The integrated cultivation-separation of plant cells is a key technology in cell culture of Taxus cuspidata Sieb.et Zucc.for the production of paclitaxel.Through the two-liquid-phase culture,effects of different organic solvents,their volume percentages,time of addition and phase toxicity on the growth of cells and yield of paclitaxel in cell culture of T.cuspidata Sieb.et Zucc.Results showed that oleic acid and dibutylphthalate were suitable organic solvents.Suitable volume percentage of organic solvents was 8%,and time for the addition of organic solvent was at day 7 to day 10.Yield of paclitaxel in the two-liquid-phase culture was four times more than that of the control.

国家教委基金 “跨世纪优秀人才培养计划”;天津市重点攻关项目 “大规模细胞培养生产紫杉醇”