建立了用HPLC法测定生物样品中马蔺子甲素和乙素的方法。以反相C18柱，乙睛-水(97:3)为流动相，紫外270nm检测，内标物为盐酸氟桂嗪。检测灵敏度为5ng/mL，回收率达75%以上，在10ng/mL～1000ng/mL浓度范围内具有良好的线性关系和重现性。体外和体内试验证明该测定方法能满足生物样品测定的要求。

An HPLC method is used to determine concentrations of irisquinone A and B in biological samples. Before analysis,the biological samples are extracted by organic solvent(n-exane:isopropanol=95: 5)transferred，evaporated and resoluted.They can be separated on reverse phase column(C¹⁸)with acetonitrile-water(93:7)as mobile phase bata flow rate of 1. 0 mL/min and detected at ultraviolet wave length of 270nm. The retention times of irisquinone A，B and internal standard (cinnarizine)are 14. 3，15. 2 and 7. 9 min, respectively. The measurements are linear over a wide concentration range (10 ng/mL-1000 ng/mL)，kand with good precision. The methd affords the sensitivity of 5 ng/ml，and the extraction recovery is 76%and 82.3%for irisquinone A and B, respectively.