[关键词]
[摘要]
用HPLC法测定蒲公英中氯原酸的含量。采用HPHypersicC18柱,200mm×2.1mm,流动相甲醇-磷酸缓冲液(23:77,流速1.0mL/min,检测波长323um。测定结果平均回收率为99.54%,RSD=2.85%。
[Key word]
[Abstract]
A method for the determination of chlorogenic acid in Herba Taraxaci was reported. Using HP Hypersic C18Column(200 mm X 2. 1 mm,5pm),with a mobile phase of methanol-potassium phosphate(23 ,77,pH4. 2),a detecting wavelength at 323 nm,and a flow rate of 1 mL/min ,the content of chlorogenic acid was found to be about 0. 5%; the recovery rate was 99. 54%, RSD 2. 85 %. The method is very stable and ac- curate.
[中图分类号]
[基金项目]