采用HPLC法测定清肝注射液中芍药甙的含量。用HypersilODS（250mm×4mmID）为色谱柱，以甲醇-异丙醇-冰醋酸-水（30：2：2：66）为流动相，检测波长为233nm。在此条件下，芍药甙的进样量在0．2μg～1．6μg时，与峰面积呈良好的线性关系（r=0．9941）。平均回收率为98．5％，方法的日内和日间精密度分别为3．46％和4．72％。

Qinggan Injection (QI) is a new Chinese medicinal preparation containing Paeonia veitchii Lynch, P.Suffruticosa Andr. and Artemisia capillaris Thumb. Paeoniflorin, the main active principle of P. vetchii,is responsible for the therapeutic effect of QI. Therefore,the determination of paeoniflorin is an effective means for the quality control of QI. A HPLC method is described for the determination of paeoniflorin in QI. A reversed phase system was used,including an ODS column with methanol-isopropanol-acetic acid-water (30:2:2:66) as the mobile phase. The column temperature was 30℃,the flow rate was 0.8 mL/min and the detection wave length was 233nm. Under this condition,the calibration curve was linear within the range from 0. 2 μg/mL～1. 6 μg/mL with r= 0.9941. The recovery of paeoniflorin in QI was 98. 5%. The relative standard deviations for within-day and between-day was 3. 46% and 4. 72%,respectively. This method is simple,rapid,sensitive,and accurate and suitable for quantitative determination of single component in compounded preparations.