本实验成功地以决明的胚轴、子叶诱导出愈伤组织。实验表明, MS比B5更利于愈伤组织诱导。正交试验表明, 添加1mg／L的IAA、6－BA、GA3可明显促进愈伤组织的生长；添加1mg／L的NAA、2, 4－D可抑制其生长；添加1mg／L的6－BA、2, 4－D对抑制愈伤组织的分化有明显的效应。适合愈伤组织生长及维持分化率低水平的蔗糖浓度为2％～3％。愈伤组织的诱导及生长周期为30d左右。

Calluses of Cassia abtusifolia I. were accessfully induced in different media. Calluses were induced betier in IVIS than in 135. Adding lmg/I1/1.A, lmyil. G-13A and lmg/IGA₃ could improve the noticeably. However,Nr A and 2.4) could depress the growth of calluses.6-BA and 2 growth of calluses 4-D showed apparent depressive effects on the differentiation of calluses.Suitable concentration of sucrose for improving the growth of calluses and lowering the differentiation of calluses was 2%-3%. The growth cycle of calluses was about 30 days.