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[摘要]
采用薄层扫描法测定复方女贞子胶囊中齐墩果酸的含量。以硅胶G为薄层吸附剂,环乙烷-丙酮-乙酸乙酯(4:2:1)为展开剂,10%硫酸乙醇为显色剂,用DS-930薄层扫描仪,在λS=530um,λR=680um波长处扫描。本法的平均回收率为99.22%,同一批样品平行试验6欢的相对标准偏差为3.14%。
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[Abstract]
Content of oleanolic acid in compound Nuzhenzi capsule was determined by TLCS on plates of silica gel G and 0. 5% CMC-Na solution. The plates were developed in a solvent system of cyclohexane-acetone-ethyl acetate(4: 2: 1 )and colored with 10% sulfuric acid-ethanol solution- Oleanolic acid was detected in the double wavelength absorbance-reflectance mode at he(530nm)and referred at AR (680 nm). The assay is linear in the range of 1. 13~9. 04 μg for oleanolic acid. Mean recovery of the assay is 99. 22%.
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