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[摘要]
用反相HPLC法测定了紫菀中槲皮素的含量。实验采用ODS柱,以0.4%磷酸溶液-甲醇(1:1)为流动相,360nm为检测波长,用外标法测定。回收率为97.39%,RSD0.39%,线性范围为8~40μg/mL。本法快速、简便、重现性好,可用于原材料的质量控制。
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[Abstract]
Rp-HPLC method was developed for the determination of quercetin in Aster tataricus I.f. . ODS column was used with a mobile phase of methanol-0.4% phosphoric acid (1:1)and detected at 360 nm. The flow rate was 1.0 mL/min. External standard was used and the calibration curve was linear over the range 8一40 μg/mL(r=0.9998).The extraction recovery was 97.39% (RSD=0.39%).The method was simple ,rapid ,and well reproducible. It can be used as a reliable method for the quality control of raw material.
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