栝楼的叶柄切段、子叶和叶片小切块在不同培养基上培养,诱导愈伤组织和不定根均较易,却不易分化不定芽.而栝楼的完整小叶和大的叶片切段及不定根尖在MS＋e-BA5mg/L培养基上均可诱导产生不定芽,不定芽经继代培养可大量繁殖.不定根尖芽在MS＋NAA0．5mg/L＋6-BA0．5mg/L培养基上壮苗培养可产生小苗,在MS＋NAA0～1mg/L培养基上可再生出完整植株,经移栽培养植株易萎蔫,成活率不高.本文还讨论了叶片的取材大小和切割方式与不定芽分化的关系.

When petiole segment,blade and cotyledon slice of Trichosanihes kililhai were cultured on MS medium with different growth regulators,callus and adventitious root were induced easily but with difficulty for adventitious bud.Yet adventitious bud can be induced when whole small blade,large blade segment and adventitious root tip were cultivated on MS medium with 0.5mg/L of 6～13A and propagated extensively when transplanted on new media for successive transfer culture.When adventitious root bud was cultivated on MS medium wth 0.5mg/L of NAA and 0.5 mg/L of 6～13A,shoot appeared,which can be grown into plantlet though the survival rate was not very satisfactory when further transplanted. Effect of blade segment size and its slicing technique,on the differentiation of adventitious bud was discussed.