蜂皇胎制品中游离赖氨酸用离子交换树脂分离后,先与铜离子络合,再与1-氟-2,4-二硝基苯(FDNB)反应,经后处理,用分光光度法测定.

Free lysine in a preparation of queen bee's embryo was isolated using cation exchange resin- The Q-aminoand carboxyl group of lysine was complexed with cupric ion and the E-amino group reacted with FDNB(Fluoro-dinitrobenzene).After acid treatment and extraction with ether,the absorbance of the solution at 395nm was determined by spectrophotometry.Variation coefficient of the determination was less than 6% and the recovery of lysine was 95% ～110%.