从川西獐牙菜的胚轴、未成熟种子及幼叶诱导出愈伤组织；试验选用MS、B₅和N₆3种培养基，其中以加有2，4－D 3mg/L＋KT0.6mg/L的MS培养基诱导率最高。结果表明，不同培养基及不同激素组合对愈伤组织诱导率有较明显的影响。初步证明了用植物组织培养的方法进行川西獐牙菜的无性快速繁殖是有可能的。

Calli were induced from young leaves,immature seeds and hypocotyl of Swertia mussotii Franch.Three kinds of media,MS,B₅ and N₆ were set up in the experiment.Calli were established with the highest frequency on to MS medium cantaining 3mg/L 2,4-D and 0.6mg/L KT.The induction andmaintenence of the calli were affected markedly by the basic media and compound 2,4-D and KT in the media which proved that asextual reproduction of S.mussotii is possible by tissue culture.