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[摘要]
采用HPLC法测定了银杏叶浸膏干粉及银杏叶胶囊中总黄酮含量。采用YWG-C18柱,甲醇-水-冰醋酸(40:57.5:2.5,V/v)为流动相,检测波长254nm。样品中10种成分分离较好以芦丁为对照外标法定量,线控范围0.5~2.5μg,r=0.9996,加样回收率为104.2%(RSD=3.3%)。
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[Abstract]
Quantitative HPLC method was developed for the determination of flavonoids in extract preparations of leaves of Ginkgo biloba L. YWG-C13 column was used with a mobile phase of methanol-water-glacial acetic acid (40: 57.5 : 2.5, V/V ) and detected at 254nm.The flow rate was 1.0ml/min. Ten Peaks were observed. Rutin was used as external standard and the calibratiott cirve was linear over the rang of 0.5~2.5μg (r =0.9996 ). The extraction recovery was 104.2% (RSD = 3.3% ) .Compared with the res ilts of chemical colorimetric analysis, the method has a better reprod icibility and more information about the flavonoids in G. biloba can be obtained.
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