用硫酸-咔唑比色法测定了红松果多糖中酸性糖的含量, 并考察了浓硫酸与咔唑的用量及反应温度、时间等因素对显色反应的影响, 最大吸收波长520nm, 测得酸性糖在1.0～7.0μg／ml浓度范围内与吸收度呈线性关系(r＝0.9999, n＝7), 平均回收率为99.0％～99.8％, RSD为1.3％～2.3％(n＝6), 最低检测浓度为0.05μg／ml。

Acid polysaccharides in pinecone were determined quantitatively by sulfuric acid-carha zole colorimetry and the influence of amount of sulfuric acid carbazole, reaction temper ature and time forcolor-reaction were investigated, The maximum abs-rption was found to be at 520nm, The abiorbance was a linear function of concentrations between o and 7 μg/ml acid polysaccharides(r=0, 9999, n=7).Analytical recovery was 91.0%~99.8%.RDS was 1, 3%~2.3%(n=6).The minimum detectable concentration was 0, 05wg/ml.