用苯酸-硫酸法进行了油松果多糖的含量测定，并考察了苯酚与浓硫酸的用量及反应温度等因素时显色反应的影响，最大吸收波长490nm，测得多糖在10～70μg/ml的浓度范围内与吸收度呈线性关系（r＝0.9999n＝6），平均回收率为99.9％，变异系数为1.2％，最低检测浓度为0.5μg/ml。

Quantitative determination of polysaccharides in Pine cones by phenol-sulfuric acid mehod was studied. and the effects of concentration of phenol-sulfuric acid and reaction temperature on color formation were investigated. It was found that the absorbance is linearly correlated to polysaceharide concentrations between 10～78μg/ml (r=0.9999). The analytical recovery was 99.8%, CV% was 1.2% and the minimal detectable concentration was 5μg/ml.