部分纯化的蛋白激酶A和蛋白激酶C，在体外分别与瑞香素温育。瑞香素浓度达0.56×10^-8mol/L时，显著抑制Ⅱ型蛋白激酶A的活力，大于0.56×10^-5mol/L时，抑制Ⅰ型蛋白激酶A的活力，而高至0.56×10^-4mol/L时，才能抑制蛋白激酶C的活力。结果表明，瑞香素对Ⅱ型蛋白激酶A有较高的特异抑制作用。

Daphnetin,isolated from Daphne koreane Nakai,has been reported to dilate blood 1s,inhibit thrombosis and have antiinflammatory effect.In this study, type I and type II protein kinase A partially purified from rat skeletal muscle and protein .kinase C from rat brain were incubated with varying concentrations of daphnetin, Type II protein kinase A was significantly inhibited by daphnetin at the concentration of 0.0056μmo1/L, while type I prot-sin kinase A and protein kinase C were inhibited at the concentrations of 5.6μmo1/Land 56μmol/L,respectively, The results indicated that daphnetin might be used as a specific inhi- bitor of type II protein kinase A.

R284.1

国家自然科学基金