目的 研究青霉素对血小板活性、寿命及黏附相关功能的影响。方法 分离C57BL/6J小鼠外周血小板，使用10、50、100、500、1 000、5000 U/mL青霉素处理24 h，MTT法检测血小板活性，计算半数抑制浓度（IC₅₀）；ELISA法检测IC₅₀的青霉素对血小板中蛋白激酶A（Protein kinase A，PKA）、β血小板球蛋白（β-thromboglobulin，β-TG）、超氧化物歧化酶2（Superoxide dismutase 2，SOD2）、血小板第4因子（Platelet factor 4，PF₄）和血小板内皮细胞黏附分子-1（Plateletendothelial cell adhesion molecule-1，PECAM-1）含量的影响。结果 随着青霉素浓度的增大，血小板数目逐渐减少，血小板存活率逐渐降低，IC₅₀值为985.68 U/mL。与对照组比较，青霉素组的PKA、β-TG、SOD2、PF₄和PECAM-1的含量显著降低，差异具有统计学意义（P<0.05）。结论 青霉素可能通过降低PKA、SOD2、β-TG、PF₄和PECAM-1含量，进而显著降低血小板存活率，抑制血小板活性及其聚集功能。

Objective To investigate the effects of penicillin on activity, longevity and adhesion-related functions of platelet. Methods Peripheral platelets of C57BL/6J mice were isolated. Platelets were treated with 10, 50, 100, 500, 1 000, 5 000 U/mL penicillin for 24 h. MTT was used to detect platelet activity and IC₅₀ values were calculated. The study was divided into control group and penicillin group. The contents of protein kinase A (PKA), β-thromboglobulin (β-TG), superoxide dismutase 2 (SOD2), Platelet factor 4 (PF4) and platelet endothelial cell adhesion molecule-1 (PECAM-1) in platelets were detected by ELISA. Results With the increase of penicillin concentration, the number of platelets decreased gradually, and the platelet survival rate gradually decreased. The calculated IC 50 value is 985.68 U/mL. Compared with the control group, the contents of PKA, β-TG, SOD2, PF4 and PECAM-1 in the penicillin group were significantly lower, and the difference was statistically significant (P<0.05). Conclusion Penicillin may significantly reduce platelet survival rate, platelet activity and aggregation function by reducing the contents of PKA, SOD2, β-TG, PF4 and PECAM-1 in platelets.

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