[关键词]
[摘要]
目的 采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS)技术对中药复方渴络欣胶囊中的化学成分进行研究。方法 采用ACQUITY UPLC HSS T3 C18色谱柱(100 mm×2.1 mm,1.8 μm),以甲醇-0.1%甲酸水溶液为流动相梯度洗脱,体积流量为0.3 mL/min;采用ESI源在正、负离子模式下分别采集数据。根据化合物精确相对分子质量及二级碎片离子信息,结合参考文献数据,鉴定渴络欣胶囊的主要化学成分。结果 从渴络欣胶囊中分离和鉴定出54个化学成分,包括蒽醌类化合物10个、黄酮类化合物10个、萜类化合物7个、酚类化合物16个等。结论 首次采用UPLC-Q-TOF/MS联用技术研究渴络欣胶囊中化学成分,进一步明确了其物质基础。
[Key word]
[Abstract]
Objective The study aims to qualitatively analyze the chemical composition of Ke-Luo-Xin Capsules extract by using UPLC-Q-TOF-MS.Methods The analysis was performed on Waters ACQUITY UPLC HSS T3 C18(100 mm×2.1 mm,1.8 μm) column.The mobile phase consisted of methanol and 0.1% formic acid-water was used as gradient elution.The flow rate was 0.3 mL·min-1 and column temperature was 35.The mass spectrometry was acquired in both positive and negative ion modes using ESI.The components were identified by the precise mass-to-charge ratio,secondary fragmentation and other information combined with reference substance and literature data.Results 54 compounds were identified,including 16 phenols,10 flavonoids and their glycosides,7 terpenoids,10 anthraquinones and so on.Conclusion The results provide reference for the study of active constituents and quality control research for Ke-Luo-Xin capsules and lay the foundation for elucidating the active components mechanism.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(81774014)